Chlorination-improved adsorption capacity of microplastics for antibiotics: A combined experimental and molecular mechanism investigation.

Microplastics and antibiotics not only pollute aquatic environments and threaten human health, but are also tricky to remove. Microplastics adsorb antibiotics, and, before being released into the natural environment, most microplastics pass through some wastewater treatment and/or disinfection (such as chlorination) facilities. It is therefore necessary to understand how these treatment processes may affect or alter microplastics' properties, particularly their ability to adsorb antibiotics, and whether or not the two, when bound together, may present exacerbated harm to the environment. This study used both laboratory tests and molecular dynamics simulation to investigate the mechanism through which chlorinated microplastics (specifically polystyrene) adsorb the antibiotic tetracycline, and showed that chlorination gave the polystyrene a larger interaction area (> 21%) and more free energy (> 14%) to adsorb tetracycline. Van der Waals (vdW) forces played a more dominant role than electrostatics in facilitating tetracycline's adsorption. Moreover, a density functional theory analysis demonstrated that the vdW potentials of the microplastics decreased as more and more hydrogen atoms became replaced by chlorine, suggesting a facilitation of the adsorption of polycyclic antibiotic molecules. The experimental results confirmed the simulation's prediction that a higher degree of chlorination significantly increases the polystyrene's adsorption capacity, whereas pH and salinity had almost no effect on the adsorption. This study demonstrates that disinfection elevates the risk of antibiotics adhering to and accumulating on the surface of microplastics.

Sulfur bacteria-reinforced microbial electrochemical denitrification.

Two limiting factors of microbial electrochemical denitrification (MED) are the abundance and efficiency of the functional microorganisms. To supply these microorganisms, MED systems are inoculated with denitrifying sludge, but such method has much room for improvement. This study compared MED inoculated with autotrophic denitrifying inoculum (ADI) versus with heterotrophic denitrifying inoculum (HDI). ADI exhibited electroactivity for 50% less of timethan HDI. The denitrification efficiency of the ADI biocathode was42% higherthan that of the HDI biocathode. The HDI biocathode had high levels of polysaccharides while the ADI biocathode was rich in proteins, suggesting that two biocathodes may achieveMED but via differentpathways. Microbial communities of two biocathodes indicated MED of HDI biocathode may rely on interspecies electron transfer, whereas sulfur bacteria of ADI biocathode take electrons directly from the cathode to achieve MED. Utilizing autotrophic sulfur-oxidizing denitrifiers, this study offers a strategy for enhancing MED.

Insights into current bio-processes and future perspectives of carbon-neutral treatment of industrial organic wastewater: A critical review.

With the rise of the concept of carbon neutrality, the current wastewater treatment process of industrial organic wastewater is moving towards the goal of energy conservation and carbon emission reduction. The advantages of anaerobic digestion (AD) processes in industrial organic wastewater treatment for bio-energy recovery, which is in line with the concept of carbon neutrality. This study summarized the significance and advantages of the state-of-the-art AD processes were reviewed in detail. The application of expanded granular sludge bed (EGSB) reactors and anaerobic membrane bioreactor (AnMBR) were particularly introduced for the effective treatment of industrial organic wastewater treatment due to its remarkable prospect of engineering application for the high-strength wastewater. This study also looks forward to the optimization of the AD processes through the enhancement strategies of micro-aeration pretreatment, acidic-alkaline pretreatment, co-digestion, and biochar addition to improve the stability of the AD system and energy recovery from of industrial organic wastewater. The integration of anaerobic ammonia oxidation (Anammox) with the AD processes for the post-treatment of nitrogenous pollutants for the industrial organic wastewater is also introduced as a feasible carbon-neutral process. The combination of AnMBR and Anammox is highly recommended as a promising carbon-neutral process for the removal of both organic and inorganic pollutants from the industrial organic wastewater for future perspective. It is also suggested that the AD processes combined with biological hydrogen production, microalgae culture, bioelectrochemical technology and other bio-processes are suitable for the low-carbon treatment of industrial organic wastewater with the concept of carbon neutrality in future.

Long-term stable and efficient degradation of ornidazole with minimized by-product formation by a biological sulfidogenic process based on elemental sulfur.

Conventional biological treatment processes cannot efficiently and completely degrade nitroimidazole antibiotics, due to the formation of highly antibacterial and carcinogenic nitroreduction by-products. This study investigated the removal of a typical nitroimidazole antibiotic (ornidazole) during wastewater treatment by a biological sulfidogenic process based on elemental sulfur (S0-BSP). Efficient and stable ornidazole degradation and organic carbon mineralization were simultaneously achieved by the S0-BSP in a 798-day bench-scale trial. Over 99.8 % of ornidazole (200‒500 µg/L) was removed with the removal rates of up to 0.59 g/(m3·d). Meanwhile, the efficiencies of organic carbon mineralization and sulfide production were hardly impacted by the dosed ornidazole, and their rates were maintained at 0.15 kg C/(m3·d) and 0.49 kg S/(m3·d), respectively. The genera associated with ornidazole degradation were identified (e.g., Sedimentibacter, Trichococcus, and Longilinea), and their abundances increased significantly. Microbial degradation of ornidazole proceeded by several functional genes, such as dehalogenases, cysteine synthase, and dioxygenases, mainly through dechlorination, denitration, N-heterocyclic ring cleavage, and oxidation. More importantly, the nucleophilic substitution of nitro group mediated by in-situ formed reducing sulfur species (e.g., sulfide, polysulfides, and cysteine hydropolysulfides), instead of nitroreduction, enhanced the complete ornidazole degradation and minimized the formation of carcinogenic and antibacterial nitroreduction by-products. The findings suggest that S0-BSP can be a promising approach to treat wastewater containing multiple contaminants, such as emerging organic pollutants, organic carbon, nitrate, and heavy metals.

From micro to macro: The role of seawater in maintaining structural integrity and bioactivity of granules in treating antibiotic-laden mariculture wastewater.

Granular sludge (GS) has superior antibiotic removal ability to flocs, due to GS's layered structure and rich extracellular polymeric substances. However, prolonged exposure to antibiotics degrades the performance and stability of GS. This study investigated how a seawater matrix might help maintain the structural integrity and bioactivity of granules. The results demonstrated that GS had better sulfadiazine (SDZ) removal efficiency in a seawater matrix (85.6 %) than in a freshwater matrix (57.6 %); the multiple ions in seawater enhanced boundary layer diffusion (kiR1 = 0.0805 mg·g-1·min-1/2 and kiR2 = 0.1112 mg·g-1·min-1/2) and improved adsorption performance by 15 % (0.123 mg/g-SS freshwater vs. 0.141 mg/g-SS seawater). Moreover, multiple hydrogen bonds (1-3) formed between each SDZ and lipid bilayer fortified the adsorption. Beyond S-N and S-C bond hydrolyses that took place in freshwater systems, there was an additional biodegradation pathway for GS to be cultivated in a saltwater system that involved sulfur dioxide extrusion. This additional pathway was attributable to the greater microbial diversity and larger presence of sulfadiazine-degrading bacteria containing SadAC genes, such as Leucobacter and Arthrobacter, in saltwater wastewater. The findings of this study elucidate how seawater influences GS properties and antibiotic removal ability.

From liquid to solid: A novel approach for utilizing sulfate reduction effluent through phase transition - Effluent-induced nanoscale zerovalent iron sulfidation.

This study investigated the use of sulfate reduction effluent (SR-effluent) to induce sulfidation on nanoscale zerovalent iron (nZVI). SR-effluent-modified nZVI achieved a 100% improvement in Cr(VI) removal from simulated groundwater, a result comparable to cases where other, more typical sulfur precursors (Na2S2O4, Na2S2O3, Na2S, K2S6, and S0) were used. Through a structural equation model analysis, amendment of nanoparticles' agglomeration (standardized path coefficient (std. path coeff.) = -0.449, p < 0.05) and hydrophobicity (std. path coeff. = 0.100, p < 0.05) and direct reaction between iron-sulfur compounds and Cr(VI) (std. path coeff. ranged from -0.195 to 0.322, p < 0.05) were primarily contributing to sulfidation-induced Cr(VI) removal enhancement. Regarding the property improvement of nZVI, the SR-effluent's corrosion radius played a crucial role in tuning the content and distribution of the iron-sulfur compounds based on the core-shell structure of the nZVI and the redox processes at the aqueous-solid interface.

Visible-light-induced strong oxidation capacity of metal-free carbon nanodots through photo-induced surface reduction for photocatalytic antibacterial and tumor therapy.

Biocompatible metal-free carbon dots (CDs) with good photo-induced strong oxidation capacity in aqueous solutions are scarce for high-performance photocatalytic antibacterial and tumor therapy. In this work, we achieved effective visible light-induced cell death and antibacterial performance based on biocompatible metal-free CDs. The visible-light-induced reducing ability of the surface electron-withdrawing structure of the CDs allowed for the remaining photo-induced holes with high oxidation capacity to oxidize water molecules and generate hydroxyl radicals. Antibiotic-resistant bacteria were effectively inhibited by the CDs under xenon lamp irradiation with 450 nm long pass filter. Moreover, CD-based tumor photocatalytic therapy in mice was achieved using a xenon lamp with 450 nm long pass filter (0.3 W cm-2).

In situ coagulation-electrochemical oxidation of leachate concentrate: A key role of cathodes.

To efficiently remove organic and inorganic pollutants from leachate concentrate, an in situ coagulation-electrochemical oxidation (CO-EO) system was proposed using Ti/Ti4O7 anode and Al cathode, coupling the "super-Faradaic" dissolution of Al. The system was evaluated in terms of the removal efficiencies of organics, nutrients, and metals, and the underlying cathodic mechanisms were investigated compared with the Ti/RuO2-IrO2 and graphite cathode systems. After a 3-h treatment, the Al-cathode system removed 89.0% of COD and 36.3% of total nitrogen (TN). The TN removal was primarily ascribed to the oxidation of both ammonia and organic-N to N2. In comparison, the Al-cathode system achieved 3-10-fold total phosphorus (TP) (62.6%) and metal removals (>80%) than Ti/RuO2-IrO2 and graphite systems. The increased removals of TP and metals were ascribed to the in situ coagulation of Al(OH)3, hydroxide precipitation, and electrodeposition. With the reduced scaling on the Al cathode surface, the formation of Al3+ and electrified Al(OH)3 lessened the requirement for cathode cleaning and increased the bulk conductivity, resulting in increased instantaneous current production (38.9%) and operating cost efficiencies (48.3 kWh kgCOD -1). The present study indicated that the in situ CO-EO process could be potentially used for treating persistent wastewater containing high levels of organic and inorganic ions.

Physicochemical changes in microplastics and formation of DBPs under ozonation.

Microplastics (MPs) are substances that pose a risk to both human life and the environment. Their types and production are increasing year on year, and their potential to cause environmental pollution is a worldwide concern. Conventional water treatment processes, particularly coagulation and sedimentation, are not effective at removing all MPs. It is therefore important to assess the morphological changes in the MPs, i.e., the thermoplastic polyurethane (TPU) and polyethylene (PE), during ozonation and the dissolved organic carbon leaching as well as chloroform formation in the subsequent chlorination. The results show that the appearance and surface chemistry of the MPs changed during the ozonation process, most notably for TPU. The trichloromethane (CHCl3) generation during chlorination was 0.168 and 0.152 µmol/L for TPU and PE, respectively, and the ozone pretreatment significantly increased the CHCl3 yield of TPU, while it had a weak effect on PE. Additional disinfection byproducts (DBPs), including CHCl2Br, CHClBr2, and CHBr3, were produced in the presence of bromide ions in the water column, and the total amount of DBPs produced by PE, PE-O, TPU, and TPU-O was significantly increased to 0.787, 0.814, 0.931, and 1.391 µmol/L, respectively. The study provides useful information for the environmental risk assessment of two representative MPs, i.e., TPU and MPs, in disinfection procedures for drinking water.

Size-Dependent Uptake and Depuration of Nanoplastics in Tilapia (Oreochromis niloticus) and Distinct Intestinal Impacts.

Nanoplastics (NPs, <1 µm) are of great concern worldwide because of their high potential risk toward organisms in aquatic systems, while very little work has been focused on their tissue-specific toxicokinetics due to the limitations of NP quantification for such a purpose. In this study, NPs with two different sizes (86 and 185 nm) were doped with palladium (Pd) to accurately determine the uptake and depuration kinetics in various tissues (intestine, stomach, liver, gill, and muscle) of tilapia (Oreochromis niloticus) in water, and subsequently, the corresponding toxic effects in the intestine were explored. Our results revealed uptake and depuration constants of 2.70-378 L kg-1 day-1 and 0.138-0.407 day-1 for NPs in tilapia for the first time, and the NPs in tissues were found to be highly dependent on the particle size. The intestine exhibited the greatest relative accumulation of both sizes of NPs; the smaller NPs caused more severe damage than the larger NPs to the intestinal mucosal layer, while the larger NPs induced a greater impact on microbiota composition. The findings of this work explicitly indicate the size-dependent toxicokinetics and intestinal toxicity pathways of NPs, providing new insights into the ecological effects of NPs on aquatic organisms.

Optimizing waste activated sludge disintegration by investigating multiple electrochemical pretreatment conditions: Performance, mechanism and modeling.

The complex and rigid floc structure often limits the reutilization of waste activated sludge (WAS). Electrochemical pretreatment (EPT) is one of the most effective technologies that can enhance WAS disintegration. But a comprehensive investigation into how multiple EPT conditions work was rarely reported. The study evaluated the effects of multiple EPT conditions, i.e., different electrolytes (NaCl, Na2SO4, and CaCl2), electrolytes dosage (0 g/L, 0.5 g/L, 1.0 g/L, and 3.0 g/L), EPT current (0 A, 0.5 A, 1.0 A, and 3.0 A) and EPT time (0 min, 30 min, 60 min, and 90 min) on WAS disintegration. The results showed that NaCl was outstanding from other electrolytes in promoting more WAS disintegration. Besides, a relatively higher NaCl dosage, a higher EPT current, and a longer EPT time promoted more reactive chlorine species (RCS), thus enhancing WAS disintegration in terms of extracellular polymeric substances (EPS) structure destruction and biodegradable organic matter release. After EPT for 60 min at NaCl dosage of 1.0 g/L and current of 1.0 A, the EPS multilayer structure destruction, biodegradable organic matters release, and soluble chemical oxygen demand (SCOD) increase in the supernatant were enhanced by 17.2 %, 130.5 %, and 238.7 %, respectively. Then a predictive quadratic model was established and the impact significance of the above EPT factors for enhancing WAS disintegration followed dosage of NaCl > current > EPT time. Furthermore, response surface methodology (RSM) suggested NaCl dosage of 2.75 g/L, current of 2.0 A, and EPT time of 30 min were the optimal EPT conditions, bringing a 42.0 % increase in the net economic benefit of WAS treatment compared to without EPT.

Effects of chlorination on microplastics pollution: Physicochemical transformation and chromium adsorption.

The large number of microplastics (MPs) that appear in the environment has drawn much attention. Few studies, however, have examined the transformation of MPs in water treatment processes and their effects on environmental pollutants. In this study, the alteration of the physicochemical characteristics of polyethylene and thermoplastic polyurethane upon chlorination, as well as the influence of this alteration on contaminants, were investigated. The findings indicated that microplastics underwent significant morphology and O-functional groups changes during chlorination. It is noteworthy that the mechanisms controlling the chlorination treatment of the two MPs are clearly different. The results of aggregation and adsorption experiments showed that the chlorination treatment enhanced the aggregation ability of the MPs in brine and their interaction with Cr(VI). The present discoveries further suggested that water treatment could alter the migration capacity of microplastics and the distribution of contaminants in the aqueous environment by altering the adsorption of microplastics to the contaminants.

Achieving superior nitrogen removal in an air-lifting internal circulating reactor for municipal wastewater treatment: Performance, kinetic analysis, and microbial pathways.

Anticipated growth in living standards has accentuated higher requirements for effluent quality from municipal wastewater treatment. In this study, an air-lifting internal circulating reactor with a high internal circulation ratio (36:1) was established to treat municipal wastewater with a long-term operation. In the bioreactor, the average effluent chemical oxygen demand, total nitrogen, and ammonium nitrogen could be 13.1, 5.7, and lower than 1 mg/L, respectively. Further analysis of nitrogen removal showed that traditional nitrification and denitrification, simultaneous nitrification and denitrification (SND), and nitrogen assimilation accounted for 27.4 %, 68.7 %, and 3.9 % respectively. The proportion of aerobic bacteria (Saprospiraceae) and facultative bacteria (Comamonadaceae) were significantly increased, indicating a higher capacity for organic degradation in the reactor. The relative abundance of denitrifying bacteria and bacterial groups with SND (Comamonadaceae) increased. These results suggested the air-lifting internal circulating reactor could be a viable and efficient option for superior nitrogen removal in wastewater treatment.

Achieving tetracycline removal enhancement with granules in marine matrices: Performance, adaptation, and mechanism studies.

Using the aerobic granular sludge (AGS) to improve tetracycline (TET) removal in the treatment of mariculture wastewater was investigated in the present study. The AGS rapidly adapted to and was sustained in seawater matrices with a robust granule strength (k = 0.0014) and a more stable sludge yield than the activated sludge (AS) (0.14 vs 0.11 g-VSS/g-CODrem). The compact structure provided the AGS with an anoxic environment, which favored the growth of N (37.3 %) and P removal bacteria (30.4 %) and the expression of functional genes (nos, nor, and nar), resulting in more than 62 % TN and TP removals, respectively. Similar abundances of aromatic compound-degrading bacteria (∼34 %) in both reactors (AGS and AS) led to comparable TET biodegradation efficiencies (∼0.045 mg/g-VSS). The greater size and surface area of the AGS expanded the boundary layer diffusion region, leading to 16 % increases in the granule's TET adsorption capacity.

Mechanistic study on the ferric chloride-based rapid cultivation and enhancement of aerobic granular sludge.

Aerobic granular sludge (AGS) can achieve simultaneous carbon, nitrogen and phosphorus removal owing to its three-dimensional oxygen gradient structure. However, long start-up period and poor operational stability restrict its application and promotion. A novel rapid granulation strategy, viz., the short-term (7 days) addition of ferric chloride at the commissioning stage, was developed and verified in this study. The granulation period was shortened by 9 days, and the formed granules were compact and dense with an Fe3+ concentration of 250 mg L-1. The addition of flocculant not only maintained a high sludge concentration during the initial stages of granulation (5.3 g L-1), but also stimulated the secretion of TB-EPS and increased protein and polysaccharide contents, thereby expediting granule formation. Additionally, ferric chloride induced a diverse microbial community in granules, resulting in the emergence of new genera, such as Thaurea, Brevundimonas and Kinneretia, which improved pollutant removal performance and flocculent aggregation. The removal efficiencies of COD, PO43--P, and NH4+-N stabilized at 94.2, 62.4, and 71.3%, respectively. Therefore, it has been demonstrated that short-term ferric chloride dosing has a synergistic effect on aerobic granulation.

Anaerobic membrane bioreactor for carbon-neutral treatment of industrial wastewater containing N, N-dimethylformamide: Evaluation of electricity, bio-energy production and carbon emission.

The feasibility of anaerobic membrane bioreactor (AnMBR) for the treatment of N, N-dimethylformamide (DMF)-containing wastewater was theoretically compared with the conventional activated sludge (CAS) process in this study. The electricity consumption and expenditure, bio-energy production and CO2 emission were investigated using the operational results of a lab-scale AnMBR operated in a long-term operation. The AnMBR was capable of producing bio-methane from wastewater and generated 3.45 kWh/m3 of electricity as recovered bio-energy while the CAS just generated 1.17 kWh/m3 of electricity from the post-treatment of excessive sludge disposal. The large quantity of bio-methane recovered by the AnMBR can also be sold as sustainable bioresource for the use of household natural gas with a theoretical profit gain of 29,821 US$/year, while that of the CAS was unprofitable. The AnMBR was also demonstrated to significantly reduce the carbon emission by obtaining a theoretical negative CO2 production of -2.34 kg CO2/m3 with the recycle of bio-energy while that for the CAS was 4.50 kg CO2/m3. The results of this study demonstrate that the AnMBR process has promising potential for the carbon-neutral treatment of high-strength DMF-containing wastewater in the future.

Biopolymer recovery from waste activated sludge toward self-healing mortar crack.

Activated sludge (AS) offers great potential for resource recovery considering its high organic and nutrient content. However, low recovery efficiency and high costs are directing the focus toward the high-valuable resource recovery. This study extracted 71.5 ± 5.9 mg/g VSS of alginate-like exopolysaccharides from AS (ALE/AS) and applied it to mortar as a novel biopolymer agent for crack self-healing. With a mortar crack of 120 µm, addition of 0.5 wt% ALE/AS yielded a high crack closure ratio of 86.5 % within 28 days. In comparison to commercial healing agents, marginal flexural strength reduction with ALE/AS addition (17.9 % vs 30.2-50.5 %) was demonstrated. The abundance of COO- group in GG blocks of ALE/AS resulted in a higher cross-link capacity with Ca2+, while the reduction of hydrophilic residues (e.g., COO- and OH) after complexation engendered a lower swelling capacity, which facilitated self-healing and flexural strength maintenance. Molecular dynamics (MD) revealed that lower Ca2+ diffusivity, arising from the stronger electrostatic interactions between the COO- groups and Ca2+, resulted in a high Ca2+ concentration around the cracks, leading to CaCO3 deposition and healed cracks. The outcomes of this study provided light on ALE-based mortar crack healing and presented a possibility for multi-level AS resource recovery.

Chemical oxygen demand and nitrogen removal from real membrane-manufacturing wastewater by a pilot-scale internal circulation reactor integrated with partial nitritation-anammox.

A pilot-scale system integrating internal circulation and partial nitritation-anammox successfully treated real high-strength membrane-manufacturing wastewater in this study. With this pilot-scale system, a high chemical oxygen demand (COD) removal efficiency of 85 % and a nitrogen removal of 90 % are achieved at an organic loading rate of 6.0 kg COD/m3/d. The nitrogenous organic matters in the internal circulation zone are degraded into ammonia nitrogen. In the partial nitrification zone, nitrite accumulation is achieved, providing a suitable NH4+-N/NO2--N ratio for anammox reaction. Partial nitritation is achieved by maintaining an operational temperature at 30-35 °C, free ammonia concentration at 5-7 mg/L and dissolved oxygen at 0.4-0.7 mg/L with a reflux ratio of 150 %. The COD to nitrogen ratio in the internal circulation effluent is maintained below 3.0 to inhibit nitrite oxidizing bacteria. This study demonstrates that a pilot-scale system can efficiently remove organic matters and nitrogen from wastewater of membrane-manufacturing industry.

Adaptive response mechanisms of granular and flocculent sulfate-reducing sludge toward acidic multi-metal-laden wastewater.

Dissimilatory sulfate reduction-based processes have long been a viable option for treating acidic metal-laden wastewater (AMW). Such processes can be optimized through enhancing sulfidogenic activity and the microbial consortia's resilience against a harsh environment. This study investigated how granular and flocculent sulfate-reducing bacteria (SRB) sludge respond to AMW as well as the mechanisms through which they adapt to the wastewater, with particular focuses on the stability of the sulfidogenic activities, metal removal, and the bacteria's resistance over the long-term: the flocculent SRB lost more than 50% of their treatment capacity after 35 days of treating AMW with the presence of Cd2+, Cu2+, Zn2+, and Ni2+ at 30 mg/L each, under pH = 4.5. In contrast, the granular SRB maintained its metal removal rate at 91% throughout the 161-day trial. Despite the SRB abundance remaining at approximate 40%, organics-partial oxidizing genera (Desulfobulbus and Desulfobacter) began to dominate due to their kinetic advantage. The extracellular glycosyl compositions were revealed to be critical for the stability of the granular structure and microbial activity as the extracellular proteins disintegrated irreversible. Usage the molecular dynamic simulation, the mobility of the metal ions in the SRB granular system was suppressed by the presence of a more diverse glycosyl composition compared with the flocculent system (10-50% diffusion coefficients differences). All of the identified glycosyls (especially xylose and rhamnose) exhibited strong interactions with Cu2+ (-470 kJ mol-1), while the maximum binding strength of Cd2+ to glycosyls was greater than -40 kJ mol-1, suggesting a low Cd2+complexation efficiency. The findings of this study shed light on the defensive mechanisms of SRB granules against multi-metal stress, and provide clues for efficient AMW treatment.

The impact of chlorination on the tetracycline sorption behavior of microplastics in aqueous solution.

Considering the large volumes of treated water and incomplete elimination of pollutants, wastewater treatment plants (WWTPs) remain a considerable source of microplastics (MPs). Chlorine, the most frequently used disinfectant in WWTPs, has a strong oxidizing impact on MPs. However, little is documented, to date, about the impact of chlorination on the transformation of MPs and the subsequent environmental behaviors of the chlorinated MPs when released into the aquatic environment. This study explored the response of the physicochemical properties of specific thermoplastics, namely polyurethane (TPU) MPs and polystyrene (PS) MPs, to chlorination and their emerging pollutant [tetracycline (TC)] adsorption behavior in aqueous solution. The results indicated that the O/C ratio of the MP surface did not significantly change, and that there were increases in the O-containing functional groups of the TPU and PS MPs, after chlorination. The surface area of the chlorinated TPU MPs increased by 45 %, and that of the chlorinated PS increased by 21 %, compared with the pristine ones, which contributed to the TC adsorption. The adsorption isotherm fitting parameters suggested that the chlorinated TPU fitted the multilayer adsorption, and the chlorinated PS was inclined to the monolayer adsorption. The relative abundance of the O-containing functional groups, on the TPU surface, led to the release of CHCl3 molecules, and the clear surface irregularities and fissures occurred after chlorine treatment. No fissures were found on the surface of the chlorinated PS MPs. The hydrophobicity and electrostatic adsorption were proved to be the major impacts on the TC adsorption of the chlorinated MPs, and the subsequently formed hydrogen bonds led to the stronger adsorption capacity of the chlorinated TPU than the chlorinated PS MPs.